The 32-year-old eight-time Olympic gold medallist is trying to transform himself from global athletics superstar to professional football player and hopes to win a contract for the Mariners ahead of the 2018-19 Australian top-flight campaign.
His previous appearance for the first team was against an amateur select side on Aug. 31 where he came on as a substitute in the 72nd minute and appeared to lack match sharpness and the touch and skill required for a professional.
"This will determine ... (what) the club wants to do with my career, so it's a very important game," Bolt said on Wednesday.
"I have been improving but you won't know what level you're at until you play a competitive game so I just have to go out there and see what I need to do or whether I should continue or not.
"I'm just pushing myself and have put in the work so now I have to go out there and execute."
Bolt said that coach Mike Mulvey had told him he was likely to start the game on Friday against Macarthur South West United at Campbelltown Sports Stadium and that he would be deployed in a more forward role than on his initial appearance.
"He said he wants me to play me up top," Bolt said.
"He just told me to make sure that I'm focussed to push myself and just take my chances when I get them."
Bolt added that he had been playing a series of practice games with other players hoping to make the first team squad and he felt that his fitness and skills had improved.
"My movement and my touch are much better now. How to set my body, where to place the ball," Bolt said.
"There is a lot of things I have learned. I am much fitter now so I will have more time on the field and that's good."
"I'm the type of guy who likes competition, I like pressure so I'm excited to go out there and give it my best," he added.
(With inputs from agencies.)