The charges relate to the irregular issuing of roadworthy certificates.
The development came hours after six-vehicle testing station employees from the same station were arrested on Monday for fraud and corruption.
"The five officials aged between 23 and 62, amongst them three examiners and two cashiers, were arrested in an ongoing investigation by the RTMC's National Traffic Anti-Corruption Unit, the Hawks and the Special Investigation Unit," the corporation said in a statement on Tuesday.
"The suspects are expected to make their first appearance at Lephalale Magistrate's Court on Wednesday to face fraud and corruption charges, along with the group that was arrested on Monday," the RTMC said.
(With Inputs from South African Government Press Release)